Small volumes of culture can be withdrawn periodically.<\/li>\n<\/ol>\nQuestion 4. \nDo eukaryotic cells have restriction endonucleases? Justify your answer. \nAnswer: \nNo. They do not have restriction endonucleases. The eukaryotic cells have some other means of defence against viral infection ie, immune system.<\/p>\n
Question 5. \nCan you recall meiosis and indicate at what stage a recombinant DNA is made? \nAnswer: \nPachytene stage of prophase I by crossing over.<\/p>\n
Question 6. \nCan you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker? \nAnswer: \nReporter enzyme can differentiate recombinants from non-recombinants on the basis of their ability to produce specific color in the presence of chromogenic substrate. ArDNA is inserted within the coding sequence of the enzyme, galactosidase.<\/p>\n
This results into inactivation f the enzyme which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue colored colonies, if the plasmid in the bacteria does not have an insert.<\/p>\n
Presence of insert results into insertional inactivation of the-galactosidase and the colonies do not produce any colour. These are identified as recombinant colonies.<\/p>\n
Plus Two Botany Biotechnology: Principles and Processes Multiple Choice Questions and Answers<\/h3>\n Question 1. \nThe linking of antibiotic resistance gene with the plasmid vector became possible with \n(a) DNA ligase \n(b) Endonucleases \n(c) DNA polymerase \n(d) Exonucleases \nAnswer: \n(a) DNA ligase<\/p>\n
Question 2. \nThe construction of the first recombinant DNA was done by using the native plasmid of \n(a) E.coli \n(b) Salmonella typhimurium \n(c) Bacillus thuringiensis \n(d) yeast \nAnswer: \n(b) Salmonella typhimurium<\/p>\n
Question 3. \nWhich of the following is used as a best genetic vector in plants? \n(a) Bacillus thuringiensis \n(b) Agrobacterium tumefaciens \n(c) Pseudomonas putida \n(d) None of the above \nAnswer: \n(b) Agrobacterium tumefaciens<\/p>\n
Question 4. \nOne of the key factors, which makes the plasmid, the vector in genetic engineering? \n(a) It is resistant to antibiotics \n(b) It is resistant to restriction enzymes \n(c) It has the ability to carry a foreign gene \n(d) It has the ability to cause infection in the host \nAnswer: \n(c) It has the ability to carry a foreign gene<\/p>\n
Question 5. \nA clone is \n(a) heterozygote obtained asexually \n(b) homozygote obtained asexually \n(c) heterozygote produced by sexual methods \n(d) homozygote produced by sexual methods \nAnswer: \n(b) homozygote obtained asexually<\/p>\n
Question 6. \nIn recombinant DNA technique, the term vector refers to \n(a) donor DNA is identified and picked up through eletrophoresis \n(b) plasmid, transfers DNA into living cell \n(c) collection of entire genome in form of plasmid \n(d) enzyme, cuts the DNA at specific sites \nAnswer: \n(b) plasmid, transfers DNA into living cell<\/p>\n
Question 7. \nThe enzyme employed for amplification of DNA during PCR is commercially obtained from \n(a) Streptococcus pyogenes \n(b) Bacillus licheniformis \n(c) Trichodermapallidum \n(d) Thermusaquaticus \nAnswer: \n(d) Thermusaquaticus<\/p>\n
Question 8. \nThe enzyme used for annealing the cut DNA segments is \n(a) DNA ligase \n(b) Helicase \n(c) Topoisomerase \n(d) Polymerase \nAnswer: \n(d) Polymerase<\/p>\n
Question 9. \nHow does PCR serve the purpose of early diagnosis? \n(a) By detecting very low amounts of DNA by amplification \n(b) It is a powerful technique to identify man genetic disorders \n(c) To detect mutations is genes in suspected cancerpatients \n(d) All the above \nAnswer: \n(d) All the above<\/p>\n
Question 10. \nEnzyme used in the addition of nucleotides in DNA replication is \n(a) catalase \n(b) DNA polymerase \n(c) nuclease \n(d) RNA polymrerase \nAnswer: \n(b) DNA polymerase<\/p>\n
Question 11. \nImportance of restriction endonuclease enzyme is to cut \n(a) RNA strands at specific site \n(b) DNA strands at specific site \n(c) Ends of DNA strands \n(d) none of the above \nAnswer: \n(b) DNA strands at specific site<\/p>\n
Question 12. \nLarge amount of recombinant protein is produced in \n(a) tissue culture \n(b) bioreactor \n(c) somatic hybridization \n(d) genetic engineering \nAnswer: \n(b) bioreactor<\/p>\n
Question 13. \nPolymerase enzyme in the replication process to produce 1 billion copies of DNA is \n(a) RNA polymerase 1 \n(b) DNA polymerase 1 \n(c) DNA polymerase 11 \n(d) taq polymerase \nAnswer: \n(d) taq polymerase<\/p>\n
Question 14. \nDigested and undigested fragments of DNA can be observed in elecrophoresis by \n(a) Ethidium bromide \n(b) CFC \n(c) Ethidium chloride \n(d) acetyl chloride \nAnswer: \n(a) Ethidium bromide<\/p>\n
Question 15. \nIn which step of DNA amplification DNA hybridization occurs \n(a) denaturation \n(b) extention \n(c) annealing \n(d) elongation \nAnswer: \n(c) annealing<\/p>\n
Question 16. \nThe plasimid of Agrobacterium is \n(a) Ti plasmid \n(b) F1 plasmid \n(c) T plasmid \n(d) col E1 \nAnswer: \n(a) Ti plasmid<\/p>\n
Question 17. \nThe ori of clonimg vector indicates \n(a) cloning site \n(b) restriction site \n(c) replication site \n(d) selectable marker site \nAnswer: \n(c) replication site<\/p>\n
Question 18. \nSelectable marker of cloning vector plays an important role in \n(a) identification of recombinants \n(b) identification of non recombinants \n(c) identification of recombinants from non recombinants \n(d) none of the above \nAnswer: \n(c) identification of recombinants from non recombinants<\/p>\n
Question 19. \nCoating of gold or tungsten particle with DNA is used in \n(a) direct gene transfer \n(b) indirect gene transfer \n(c) both direct and indirect gene transfer \n(d) none of the above \nAnswer: \n(a) direct gene transfer<\/p>\n
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Plus Two Botany Chapter Wise Questions and Answers Chapter 4 Biotechnology Principles and Processes is part of Plus Two Botany Chapter Wise Questions and Answers. Here we have given Plus Two Botany Chapter Wise Questions and Answers Chapter 4 Biotechnology Principles and Processes. Board SCERT, Kerala Text Book NCERT Based Class Plus Two Subject Botany […]<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"categories":[42728],"tags":[48265],"yoast_head":"\n
Plus Two Botany Chapter Wise Questions and Answers Chapter 4 Biotechnology Principles and Processes - A Plus Topper.com<\/title>\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n\t \n\t \n\t \n